Personnel / Charlotte

Name: Dr. Charlotte Morrison
Position: Research Associate
Email: chmorris@interchange.ubc.ca

Research Interests: Cellular activation pathways of MMPs. Mammalian protein expression and trafficking.

University of British Columbia Graduate Fellowship (full) (1994-1997)

Graduate Studies Travel Award (1997)

The Biotechnology Laboratory Travel Award (1996)

Refereed Journal Publications

Morrison, C.J., Butler, G.S., Bigg, H.F., Roberts, C.R, Soloway, P.D., and Overall, C.M. (2001).Cellular activation of MMP-2 (gelatinase A) by MT2-MMP occurs in a TIMP-2 independent pathway. J. Biol. Chem. (2001) 276 47402-47410

Bigg, H.F., Morrison, C.J., Butler, G.S., Bogoyevitch, M.A., Wang, Z., Soloway, P.D., and Overall, C.M. (2001) Tissue inhibitor of metalloproteinase-4 (TIMP-4) suppresses TIMP-2-mediated activation of progelatinase A via efficient inhibition of membrane type-1 metalloprorteinase, but cannot support progelatinase A activation. Cancer Research 61(9): 3610-3618

Overall, C.M., Tam, E., McQuibban, G.A., Morrison, C.J., Wallon, U.M., Bigg, H.F., King, A.E. and Roberts, C.R. (2000) Domain interactions in the gelatinase A.TIMP-2.MT1-MMP activation complex: The ectodomain of the 44-Kda form of the membrane type-1 metalloprorteinase does not modulate gelatinase A activation. Journal of Biological Chemistry 275: 39497-39506

Morrison, C.J., Easton, R.L., Morris, H.R., McMaster, W.R., Piret, J.M. and Dell, A. (2000). Modification of a recombinant GPI-anchored metalloproteinase for secretion alters protein glycosylation. Biotechnology and Bioengineering 68: 407-421.

Morrison, C.J., McMaster, W.R. and Piret, J.M. (1996). Differential stability of proteolytically active and inactive recombinant metalloproteinase in Chinese hamster ovary cells. Biotechnology and Bioengineering 53: 594-600.

Macdonald, M.H., Morrison, C.J. and McMaster, W.R. (1995). Analysis of the Active Site and Activation Mechanism of the Leishmania Surface Metalloproteinase GP63. Biochemistry Biophysics Acta 1253:199-207

Brittingham, A., Morrison, C.J., McMaster, W.R., McGwire, B., Chang, K.P. and Mosser, D.M. (1995). The role of the Leishmania surface proteinase GP63, in complement fixation, cell adhesion and resistance to complement mediated cell lysis. Journal of Immunology 155: 3102-3111

McMaster, W.R., Morrison, C.J., Macdonald, M.H. and Joshi, P. (1994). Mutational and functional analysis of the Leishmania surface metalloproteinase GP63: Similarities to other matrix metalloproteinases. Parasitology 108: 29-36

Kettleborough, C.A., Saldanha, J., Heath, V.J., Morrison, C.J. and Bendig, M.M. (1991). Humanization of a mouse monoclonal antibody by CDR-grafting: the importance of framework residues on loop conformation. Protein Engineering 4: 773-783.

Non-refereed contributions

McMaster, W.R., Button, L., Webb, J., Morrison, C.J. and Macdonald, M. (1992). Expression and function of the GP63 protease of Leishmania. Journal of Cellular Biochemistry. supplement 16A: 111 (abstract)

Morrison, C.J. and Sanders, P. (1989). Study of two hybridoma cell lines. In: Spier, R.E., Griffiths, J.B., Stephenne, J. and Crooy P.J. (Eds.). Advances in animal cell biology and technology for bioprocesses.Butterworths, Sevenoaks, Kent.

Conference Presentations

Morrison, C.J., Butler, G.S., Bigg, H.F., Roberts, C.R, Soloway, P.D., and Overall, C.M. Activation of gelatinase A by MT2-MMP in a TIMP-2 independent pathway and the role of TIMP-4 in gealatinase A activation. MMP Gordon Conference May 2001

Morrison, C.J., Easton, R.L., Dell, A., Morris, H.R., McMaster, W.R. and Piret, J.M. Modification of a recombinant metalloproteinase for secretion in Chinese hamster ovary (CHO) cells alters post-translational processing. The American Chemical Society meeting April 1997

Morrison, C.J., McMaster, W.R., and Piret, J.M. Enhanced stability of expression of a recombinant membrane metalloproteinase in Chinese hamster ovary cells. The American Chemical Society meeting March 1996

Poster Presentations:

Morrison, C.J., Easton, R.L., Dell, A., Morris, H.R., McMaster, W.R. and Piret, J.M. Differences in the post-translational modifications of secreted and membrane forms of a recombinant metalloproteinase expressed in Chinese hamster ovary (CHO) cells. Keystone symposium March 1997

Morrison, C.J., McMaster, W.R., and Piret, J.M. Stability of CHO Cells Expressing a Recombinant Membrane Protein. The Engineering Foundation conference January 1996

Name: Dr. Charlotte Morrison Position: Research Associate Email: chmorris@interchange.ubc.ca Research Interests: Cellular activation pathways of MMPs. Mammalian protein expression and trafficking.	Education and Qualifications
	Ph.D. in Microbiology & Immunology (1992-1997) University of British Columbia Thesis : "Post-translational modifications and expression stability of GPI-anchored and secreted forms a recombinant metalloproteinase
	BSc. Hons. Microbiology (1982-1986) University of Surrey, Guildford, Surrey, UK
	Awards
	University of British Columbia Graduate Fellowship (full) (1994-1997) Graduate Studies Travel Award (1997) The Biotechnology Laboratory Travel Award (1996)
	Publications
	Refereed Journal Publications
	Morrison, C.J., Butler, G.S., Bigg, H.F., Roberts, C.R, Soloway, P.D., and Overall, C.M. (2001).Cellular activation of MMP-2 (gelatinase A) by MT2-MMP occurs in a TIMP-2 independent pathway. J. Biol. Chem. (2001) 276 47402-47410
	Bigg, H.F., Morrison, C.J., Butler, G.S., Bogoyevitch, M.A., Wang, Z., Soloway, P.D., and Overall, C.M. (2001) Tissue inhibitor of metalloproteinase-4 (TIMP-4) suppresses TIMP-2-mediated activation of progelatinase A via efficient inhibition of membrane type-1 metalloprorteinase, but cannot support progelatinase A activation. Cancer Research 61(9): 3610-3618
	Overall, C.M., Tam, E., McQuibban, G.A., Morrison, C.J., Wallon, U.M., Bigg, H.F., King, A.E. and Roberts, C.R. (2000) Domain interactions in the gelatinase A.TIMP-2.MT1-MMP activation complex: The ectodomain of the 44-Kda form of the membrane type-1 metalloprorteinase does not modulate gelatinase A activation. Journal of Biological Chemistry 275: 39497-39506
	Morrison, C.J., Easton, R.L., Morris, H.R., McMaster, W.R., Piret, J.M. and Dell, A. (2000). Modification of a recombinant GPI-anchored metalloproteinase for secretion alters protein glycosylation. Biotechnology and Bioengineering 68: 407-421.
	Morrison, C.J., McMaster, W.R. and Piret, J.M. (1996). Differential stability of proteolytically active and inactive recombinant metalloproteinase in Chinese hamster ovary cells. Biotechnology and Bioengineering 53: 594-600.
	Macdonald, M.H., Morrison, C.J. and McMaster, W.R. (1995). Analysis of the Active Site and Activation Mechanism of the Leishmania Surface Metalloproteinase GP63. Biochemistry Biophysics Acta 1253:199-207
	Brittingham, A., Morrison, C.J., McMaster, W.R., McGwire, B., Chang, K.P. and Mosser, D.M. (1995). The role of the Leishmania surface proteinase GP63, in complement fixation, cell adhesion and resistance to complement mediated cell lysis. Journal of Immunology 155: 3102-3111
	McMaster, W.R., Morrison, C.J., Macdonald, M.H. and Joshi, P. (1994). Mutational and functional analysis of the Leishmania surface metalloproteinase GP63: Similarities to other matrix metalloproteinases. Parasitology 108: 29-36
	Kettleborough, C.A., Saldanha, J., Heath, V.J., Morrison, C.J. and Bendig, M.M. (1991). Humanization of a mouse monoclonal antibody by CDR-grafting: the importance of framework residues on loop conformation. Protein Engineering 4: 773-783.
	Non-refereed contributions
	McMaster, W.R., Button, L., Webb, J., Morrison, C.J. and Macdonald, M. (1992). Expression and function of the GP63 protease of Leishmania. Journal of Cellular Biochemistry. supplement 16A: 111 (abstract)
	Morrison, C.J. and Sanders, P. (1989). Study of two hybridoma cell lines. In: Spier, R.E., Griffiths, J.B., Stephenne, J. and Crooy P.J. (Eds.). Advances in animal cell biology and technology for bioprocesses.Butterworths, Sevenoaks, Kent.
	Conference Presentations
	Morrison, C.J., Butler, G.S., Bigg, H.F., Roberts, C.R, Soloway, P.D., and Overall, C.M. Activation of gelatinase A by MT2-MMP in a TIMP-2 independent pathway and the role of TIMP-4 in gealatinase A activation. MMP Gordon Conference May 2001
	Morrison, C.J., Easton, R.L., Dell, A., Morris, H.R., McMaster, W.R. and Piret, J.M. Modification of a recombinant metalloproteinase for secretion in Chinese hamster ovary (CHO) cells alters post-translational processing. The American Chemical Society meeting April 1997
	Morrison, C.J., McMaster, W.R., and Piret, J.M. Enhanced stability of expression of a recombinant membrane metalloproteinase in Chinese hamster ovary cells. The American Chemical Society meeting March 1996
	Poster Presentations:
	Morrison, C.J., Easton, R.L., Dell, A., Morris, H.R., McMaster, W.R. and Piret, J.M. Differences in the post-translational modifications of secreted and membrane forms of a recombinant metalloproteinase expressed in Chinese hamster ovary (CHO) cells. Keystone symposium March 1997
	Morrison, C.J., McMaster, W.R., and Piret, J.M. Stability of CHO Cells Expressing a Recombinant Membrane Protein. The Engineering Foundation conference January 1996